CS Nunemaker, RA DeFazio… - Biological …, 2003 - biologicalproceduresonline …
Excitable cells in many endocrine and neuronal systems display rhythms with periodicities
on the order of many minutes. To observe firing patterns that represent the output of these
rhythms requires a recording technique that can monitor electrophysiological activity for
several hours without affecting cell behavior. A targeted extracellular approach (also known
as loose-patch) accomplishes this objective. Because low resistance seals (< 20 MΩ) do not
influence the cell membrane and because the normal intracellular milieu is maintained, this …